Optimization of nitrogen removal conditions based on response surface methodology and nitrogen removal pathway of
Yuhong Zhang1, Jiaqi Xu1, Xianbo Dong1
1College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China.
Abstract:
The strain Paracoccus sp. QD-19 was isolated from the sludge-water mixture of aerobic tanks at the southern wastewater treatment plant in Shenyang, China. The optimal nitrogen removal conditions for strain QD-19 were determined using the Plackett-Burman design, path of steepest ascent method, and response surface methodology (RSM). The optimum nitrogen removal conditions were C/N 12.93, temperature 37 °C, and shaking speed 175.50 r/min. Strain QD-19 achieved 83.82 ± 0.80 % nitrogen removal efficiency at 10 h under optimum conditions. Functional enzyme-encodinge genes amplified via 16S rRNA sequence analysis included amoA, hao, napA, nirS, nirK, norB, and nosZ. The results revealed that NH4+-N → NH2OH → NO2--N → NO3--N → NO2--N → NO → N2O → N2 was the pathway for heterotrophic nitrification - aerobic denitrification. The strain was used to treat wastewater from a sewage treatment plant under optimal response surface methodology conditions. As a result, the TN removal efficiency was 77.11 %. The findings demonstrated that strain QD-19 exhibits favorable potential for heterotrophic nitrification and aerobic denitrification (HN-AD) of actual wastewater, presenting a promising application for biological wastewater treatment.
