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Updated: Jul 11, 2025

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

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Red edge excitation shift spectroscopy is highly sensitive to tryptophan composition

Annmaree K Warrender1, Jolyn Pan2, Chris Pudney3

  • 1Te Huataki Waiora School of Health, University of Waikato, Hamilton, New Zealand.

Journal of the Royal Society, Interface
|November 7, 2023
PubMed
Summary

Red edge excitation shift (REES) spectroscopy reveals protein dynamics. This study shows REES is highly sensitive to minor changes in protein structure and tryptophan content, impacting molecular dynamics analysis.

Keywords:
antibodiesprotein dynamicsprotein stabilityred edge excitation shifttryptophan fluorescence

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