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Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

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Published on: September 6, 2024

Biocompatible Rhamnolipid Self-Assemblies with pH-Responsive Antimicrobial Activity.

Parth Kadakia¹, Jules D P Valentin¹, Linda Hong¹

¹Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland.

Advanced Healthcare Materials

|November 7, 2023

Summary

A new biocompatible material combining rhamnolipids and glycerol monooleate shows pH-switchable antimicrobial activity. This novel material effectively targets bacteria like Staphylococcus aureus at acidic pH, offering a potential alternative to conventional antibiotics.

Keywords:

amphiphilic lipids antibacterial nanomaterials pH-triggered self-assembly rhamnolipids small-angle X-ray scattering

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Last Updated: Jul 11, 2025

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Published on: February 27, 2019

Area of Science:

Biomaterials Science
Supramolecular Chemistry
Antimicrobial Research

Background:

Growing antibiotic resistance necessitates novel antimicrobial strategies.
Development of biocompatible materials for targeted bacterial infection treatment is crucial.
Lipid-based self-assembly offers a platform for advanced functional materials.

Purpose of the Study:

To create a pH-switchable antimicrobial material using rhamnolipids (RL) and glycerol monooleate (GMO).
To investigate the structure-activity relationship of RL/GMO supramolecular dispersions.
To optimize the material for targeting bacteria in acidic wound environments.

Main Methods:

Synthesis and characterization of RL/GMO supramolecular dispersions.
Small-angle X-ray scattering (SAXS) and cryogenic transmission electron microscopy (cryo-TEM) for structural analysis.
Dynamic light scattering (DLS) for colloidal stability assessment.
Antimicrobial efficacy testing against Staphylococcus aureus and MRSA at varying pH.

Main Results:

RL/GMO dispersions exhibit a pH-triggered inverse-type liquid crystalline cubic structure.
Effective reduction of Staphylococcus aureus populations (7-log) and prevention of biofilm formation at pH 5.0.
Activity against MRSA with a minimum inhibitory concentration of 128 µg mL⁻¹ at pH 5.0.
No significant activity observed against Gram-negative bacteria or at neutral pH.

Conclusions:

A biocompatible, pH-switchable antimicrobial material was successfully developed.
The study provides fundamental insights into lipid self-assembly for biomaterial design.
This work guides the development of bio-based alternatives to combat antibiotic-resistant bacteria.