An efficient biosensor using a functionalized microneedle of Cu₂O-based CoCu-LDH for glucose detection

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biosensing

Background:

• Accurate glucose detection is crucial for personal health monitoring, especially with small sample volumes.
• Developing portable, minimally invasive, and cost-effective glucose sensing platforms remains a significant challenge.

Purpose of the Study:

• To develop a highly sensitive and stable non-enzymatic electrochemical glucose sensor.
• To utilize a novel composite material for enhanced electrocatalytic activity and electron transport.

Main Methods:

• Electrochemical deposition of copper oxide nanoparticles (Cu2O NPs) onto a stainless steel acupuncture needle electrode (ANE).
• Further preparation of cobalt-copper layered double hydroxide (CoCu-LDH) nanosheets on the Cu2O/ANE surface.
• Characterization of the composite material using FE-SEM, EDS, XRD, FT-IR, and TEM.

Main Results:

• The CoCu-LDH/Cu2O/ANE sensor demonstrated dual linear ranges for glucose detection (0.03-0.40 mM and 0.40-6.00 mM) with high sensitivities.
• Achieved a low detection limit of 0.46 μM, a rapid response time of 3 seconds, and excellent stability over 15 days.
• The sensor showed stable performance in the presence of common interfering substances like ascorbic acid and dopamine.

Conclusions:

• The unique morphology and synergistic effects of CoCu-LDH/Cu2O composites enhance glucose electrooxidation.
• The developed sensor is effective for glucose analysis in human serum, validating its potential for practical applications.
• This work presents an attractive strategy for creating portable, minimally invasive, and low-cost electrochemical glucose sensing platforms.