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Fructooligosaccharides and fructans from Platycodon grandiflorum: Structural characterization, lung-oriented guidance
Jun Liang1, Wen-Fei Wang2, Yi Zhang1
1Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Harbin 150040, China.
Carbohydrate Polymers
|November 8, 2023
Summary
Platycodon grandiflorum fraction Fr. B guides therapeutics to lungs by activating the Golgi-ER transport system. It targets Rab21, enhancing uptake of substances into lung cells.
Area of Science:
- Pharmacology and Traditional Chinese Medicine (TCM)
- Molecular Biology and Cellular Transport
Background:
- Platycodon grandiflorum (PG) is utilized in TCM as a conductant drug, but its lung-targeting mechanism is unclear.
- Traditional Chinese Medicine (TCM) seeks to enhance drug delivery to specific organs, particularly the lungs.
Purpose of the Study:
- To elucidate the mechanism by which a Platycodon grandiflorum fraction (Fr. B) achieves lung-oriented guidance.
- To identify the molecular targets responsible for Fr. B's ability to direct therapeutic substances to the lungs.
Main Methods:
- Preparation of an ethanol-soluble fraction (Fr. B) from Platycodon grandiflorum.
- Component identification using HILIC-ESI-MS/MS, MS/MS, and 1/2D NMR.
- Investigation of Fr. B's interaction with the Golgi-ER transport system and Rab21 protein via knockdown and overexpression studies.
Main Results:
- Fr. B comprises fructooligosaccharides and small molecular weight fructans.
- Fr. B activates the Golgi-ER transport system, demonstrating lung-oriented targetability.
- Rab21, a lung-specific transmembrane protein, is identified as the core target of Fr. B, facilitating extracellular substance uptake.
Conclusions:
- Platycodon grandiflorum fraction Fr. B facilitates lung-specific drug delivery.
- The mechanism involves direct interaction with Rab21 and stimulation of the Golgi-ER transport system.
- This study provides a molecular basis for the lung-targeting efficacy of Platycodon grandiflorum in TCM.
