Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

23
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
23
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

21
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
21
Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

1.0K
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
1.0K
Assessing Body Temperature - Tympanic membrane01:14

Assessing Body Temperature - Tympanic membrane

591
Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
Step 1: Begin by practicing good hand hygiene to prevent the transmission of microorganisms.
Step 2: Turn on the thermometer and wait until the ready sign appears on the screen to ensure accurate measurement.
Step 3: Slide the probe cover in place to prevent cross-contamination.
Step 4: Instruct the patient to tilt their head to the side for comfort and check for cerumen...
591
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

37
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
37
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

39
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
39

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood management with preoperative autologous blood in valve-sparing root replacement: separated autologous red blood cells and plasma and fibrin sealant versus autologous whole blood.

General thoracic and cardiovascular surgery·2026
Same author

Contribution of the Serotonin 5-HT<sub>2A</sub> Receptor to the Therapeutic Effect of Psilocin on Social Behavior Deficits in Mice Repeatedly Exposed to Social Defeat Stress.

Neuropsychopharmacology reports·2026
Same author

Self-reported POCUS practice and confidence after structured workshop for early-career nephrologists: a questionnaire study.

Clinical and experimental nephrology·2026
Same authorSame journal

Increased abundance of Limosilactobacillus reuteri in the gut of selectively bred high-tameness mice and its association with behavioural changes.

DNA research : an international journal for rapid publication of reports on genes and genomes·2026
Same author

Beckwith-Wiedemann spectrum exhibiting a 46,XY karyotype caused by genome-wide paternal uniparental heterodisomy: a case report.

Human genomics·2026
Same author

Evaluating Thioredoxin-Mediated CF<sub>o</sub>CF<sub>1</sub> Reduction Using an In Vitro Thylakoid Assay.

Bio-protocol·2026

Related Experiment Video

Updated: Jul 11, 2025

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
10:08

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

17.2K

Metagenomic Thermometer.

Masaomi Kurokawa1, Koichi Higashi1,2, Keisuke Yoshida2

  • 1Genome Evolution Laboratory, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
|November 8, 2023
PubMed
Summary

Scientists developed a Metagenomic Thermometer to predict environmental temperatures using microbial genome data. This tool analyzes amino acid fractions to determine temperature, offering insights into microbial community assembly.

Keywords:
community assemblyhot springhuman gutmetagenometemperature

More Related Videos

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
09:11

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Published on: April 4, 2021

3.2K
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.7K

Related Experiment Videos

Last Updated: Jul 11, 2025

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
10:08

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

17.2K
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
09:11

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Published on: April 4, 2021

3.2K
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.7K

Area of Science:

  • Microbiology
  • Genomics
  • Environmental Science

Background:

  • Microorganisms thrive at specific optimal growth temperatures (OGTs).
  • Previous studies linked prokaryotic OGT to genomic amino acid composition (IVYWREL).

Purpose of the Study:

  • To develop and validate a 'Metagenomic Thermometer' for predicting environmental temperatures from metagenomic data.
  • To explore the influence of temperature on microbial community structure.

Main Methods:

  • Utilized a previously identified correlation between amino acid fractions (IVYWREL) and OGT.
  • Applied the Metagenomic Thermometer to analyze publicly available metagenomic datasets.
  • Validated the tool through an experiment with an artificial hot water canal and human gut samples.

Main Results:

  • The Metagenomic Thermometer accurately predicted temperatures in diverse environments, sensitive to small changes.
  • Demonstrated accuracy in controlled (hot water canal) and complex (human gut) settings.
  • Human gut temperature correlated with dominant microbial community lineage, suggesting temperature's role in assembly.

Conclusions:

  • The Metagenomic Thermometer offers a novel method for inferring environmental temperatures from metagenomic sequences.
  • Amino acid composition, rather than microbial taxa, can drive community assembly.
  • This approach provides new insights into temperature's ecological impact on microbial communities.