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Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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A medium-density genotyping platform for cultivated strawberry using DArTag technology.

Michael A Hardigan1,2, Mitchell J Feldmann2, Jason Carling3

  • 1USDA-ARS, Horticultural Crops Production and Genetic Improvement Research Unit, Corvallis, Oregon, USA.

The Plant Genome
|November 8, 2023
PubMed
Summary

We developed a low-cost DArTag genotyping platform using molecular inversion probes (MIPs) for efficient strawberry genomic prediction in large breeding populations. This high-throughput solution enables accurate marker calling and analysis of genetic diversity.

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Area of Science:

  • Plant genetics
  • Genomic prediction
  • Strawberry breeding

Background:

  • Genomic prediction in strawberry is costly with current high-density marker platforms.
  • Breeding populations require cost-effective, high-throughput genotyping solutions.

Purpose of the Study:

  • To develop and evaluate a mid-density molecular inversion probe (MIP) panel for the DArTag platform.
  • To provide a low-cost, high-throughput genotyping solution for strawberry genomic prediction.

Main Methods:

  • Designed MIP assays targeting 7742 single nucleotide polymorphism (SNP) regions.
  • Tested assays on 376 octoploid Fragaria accessions.
  • Evaluated DArTag performance using genotype segregation, amplicon coverage, and subgenome-specific alignments.

Main Results:

  • Developed 3K and 5K DArTag panels with high polymorphism in diverse strawberry germplasm.
  • Demonstrated accurate genotype calling with low amplification bias.
  • Showed marker genotypes can be treated as functionally diploid for quantitative genetic analysis.

Conclusions:

  • The DArTag platform offers a cost-effective and efficient genotyping solution for strawberry genomic prediction.
  • The developed panels facilitate large-scale genotyping and analysis of genetic diversity in strawberry breeding programs.