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Related Concept Videos

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Related Experiment Video

Updated: Jul 11, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
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High-resolution open-top axially swept light sheet microscopy.

Soohyun Park1, Myeongsu Na2, Sunghoe Chang3,4

  • 1Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk, 37673, Republic of Korea.

BMC Biology
|November 8, 2023
PubMed
Summary
This summary is machine-generated.

A new high-resolution open-top axially swept light-sheet microscopy (HR-OTAS-LSM) enables submicron imaging for large biological specimens like the brain. This technique advances high-throughput cellular network mapping in neuroscience research.

Keywords:
Aberration correctionAxial sweptLight sheet microscopyOpen-topOptically cleared tissue

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Area of Science:

  • Biomedical Engineering
  • Microscopy Techniques
  • Neuroscience Imaging

Background:

  • Open-top light-sheet microscopy (OT-LSM) is crucial for high-throughput imaging of large, cleared biological specimens.
  • Current OT-LSM methods face challenges in achieving submicron resolution across all dimensions.

Purpose of the Study:

  • To develop a novel high-resolution OT-LSM (HR-OTAS-LSM) system.
  • To achieve simultaneous high-throughput and submicron resolution imaging in all dimensions.

Main Methods:

  • Utilized an aberration-corrected axially swept excitation light sheet for high axial resolution.
  • Employed a high numerical aperture (NA) immersion objective lens for high lateral resolution.
  • Developed a high-resolution open-top axially swept light-sheet microscopy (HR-OTAS-LSM) system.

Main Results:

  • The HR-OTAS-LSM achieved high resolution in both axial and lateral dimensions.
  • Demonstrated high-throughput visualization of neuronal networks in mouse brain specimens.
  • Validated performance with high-resolution imaging of mouse retina specimens.

Conclusions:

  • The HR-OTAS-LSM system represents a significant advancement in microscopy.
  • Enables high-resolution mapping of cellular networks in biological systems like the brain and retina.