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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 11, 2025

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

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Guidelines for establishing a cytometry laboratory.

Anna C Belkina¹, Caroline E Roe², Vera A Tang³

¹Flow Cytometry Core Facility, School of Medicine, Boston University, Boston, Massachusetts, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|November 9, 2023

Summary

This summary is machine-generated.

This guide offers best practices for establishing and managing flow cytometry shared resource laboratories (SRLs). It covers operational excellence, technology, and staffing to meet scientific community needs.

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Area of Science:

Biotechnology
Laboratory Management
Scientific Instrumentation

Background:

Flow cytometry shared resource laboratories (SRLs) are crucial for advancing biological research.
Effective management and operational excellence are key to maximizing the utility of these specialized facilities.
Previous guidelines provide a foundation, but advancements necessitate updated best practices.

Purpose of the Study:

To provide comprehensive guidance for establishing and maintaining flow cytometry SRLs.
To outline prioritized goals for operational excellence and meeting scientific community needs.
To detail relevant technologies, software, operational requirements, and management strategies.

Main Methods:

Compilation of expertise from the International Society for Advancement of Cytometry (ISAC) Shared Resource Laboratory (SRL) Emerging Leaders Program.
Review of recent advancements in cytometric technology and management practices.
Incorporation of best practices for housing, operation, and support of flow cytometry equipment.

Main Results:

Key goals for prioritizing operational excellence in flow cytometry SRLs are outlined.
Information on available cytometry technologies and relevant software is provided.
Best practices for staffing, management, and operational requirements are detailed.

Conclusions:

This resource assists in establishing new flow cytometry SRLs, repurposing existing spaces, or integrating flow cytometers into individual labs.
Adherence to best practices ensures efficient operation and continued support for the scientific community.
The guide emphasizes the importance of careful planning regarding location, space, resources, and support for successful SRL operation.