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Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

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An image-based Abplex method for high-throughput GPCRs antibody discovery.

Min Jiang^1,2, Yuanyuan Wang², Xinke Yu³

¹School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, China.

Biotechnology Journal

|November 9, 2023

Summary

This summary is machine-generated.

We developed Abplex, a fast, high-content imaging antibody screening system for membrane proteins like G protein-coupled receptors (GPCRs). This method uses intact cells and cell barcoding for robust, efficient antibody discovery and therapeutic development.

Keywords:

Abplex GPCRs antibody discovery cell barcode

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Area of Science:

Biotechnology
Drug Discovery
Molecular Biology

Background:

Membrane proteins, especially G protein-coupled receptors (GPCRs), are critical drug targets.
Extracting membrane proteins for antibody screening is challenging.
Existing methods like CbELISA and FACS have limitations in throughput and efficiency.

Purpose of the Study:

To develop an innovative antibody screening strategy, Abplex, for membrane proteins.
To leverage high-content imaging and intact cells for native protein presentation.
To enhance antibody screening robustness, speed, and throughput.

Main Methods:

Developed Abplex, an antibody screening strategy using high-content imaging.
Utilized intact cells expressing target membrane proteins to maintain native conformation.
Incorporated cell barcoding for multiplexed detection and analysis.
Acquired specific and non-specific binding signals within a single well.

Main Results:

Abplex offers a simplified workflow with reduced hands-on time (50 min total).
Achieved rapid analysis with single-sample processing in one second.
Demonstrated superior throughput and precision compared to traditional methods.
Enabled multiplexed detection through cell barcoding.

Conclusions:

Abplex significantly expedites G protein-coupled receptor (GPCR)-targeting antibody discovery.
The method enhances the development of antibody therapeutics.
Abplex promises to advance new disease treatments through improved antibody screening.