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Updated: Jul 11, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

The multiple activations in budding yeast S-phase checkpoint are Poisson processes.

Xin Gao¹, Peijie Zhou², Fangting Li¹

¹School of Physics, Center for Quantitative Biology, Peking University, Beijing 100871, China.

|November 9, 2023

Summary

Cell cycle checkpoints activate stochastically due to DNA replication stress. This study reveals the S-phase checkpoint activation follows a Poisson process, offering insights into cellular signaling dynamics.

Keywords:

Poisson activation process S-phase checkpoint budding yeast stochasticity

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Published on: October 21, 2022

Area of Science:

Cell Biology
Biophysics
Molecular Biology

Background:

Eukaryotic cells possess a DNA replication checkpoint to manage DNA replication stress.
Biochemical reaction noise causes cell-to-cell variability in checkpoint activation.
Understanding stochasticity in cell cycle regulation is crucial.

Purpose of the Study:

To investigate the stochastic nature of S-phase checkpoint activation in budding yeast.
To determine the statistical distribution governing multiple checkpoint activations within a single cell cycle.
To elucidate the dynamics of signal transduction pathways under replication stress.

Main Methods:

Microfluidics-integrated time-lapse imaging of budding yeast.
Single-cell level dynamic analysis.
Mathematical modeling of checkpoint activation events.
Experimental validation using mutant strains.

Main Results:

Observed multiple S-phase checkpoint activations within a single cell cycle, varying in timing and number.
Stochastic waiting times between activations are exponentially distributed and independent.
The number of activations under varying replication stress levels follows a Poisson distribution.
Finite DNA replication time imposes an upper limit on multiple activations.

Conclusions:

S-phase checkpoint activation events during DNA replication exhibit characteristics of a Poisson process.
This stochastic activation provides a new perspective on the complex dynamics of cellular signal transduction.
The findings contribute to understanding cellular responses to DNA replication stress.