Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 11, 2025

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

13.2K

Detecting and differentiating neurotransmitters using ultraviolet plasmonic engineered native fluorescence.

Ji-Young Lee1, Mohammad Mohammadi1, Yunshan Wang1

  • 1Department of Chemical Engineering, University of Utah Salt Lake City 84112 USA yunshan.wang@utah.edu.

RSC Advances
|November 9, 2023
PubMed
Summary

This study introduces ultraviolet (UV) plasmonic engineered native fluorescence for sensitive and selective neurotransmitter detection. This new method enhances fluorescence and reduces photobleaching, improving detection accuracy for molecules like dopamine.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Theory of Mind and Information Processing Speed in Multiple Sclerosis: Associations With Personality Traits.

Brain and behavior·2026
Same author

Pathways Mediating Blindsight After V1 Injury: A Systematic Review of Human and Monkey Studies.

Basic and clinical neuroscience·2026
Same author

Perceived professional preparedness of final-year nursing students in Iran: a cross-sectional study.

BMC nursing·2026
Same author

Bone Mineral Density Loss in Relapsing-remitting Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder: A Matched Case-control Study.

International journal of preventive medicine·2026
Same author

Loss of Consciousness in a Child Following Accidental Ingestion of Brimonidine Ointment: A Case Report.

Clinical case reports·2026
Same author

An interpretable approach to detect case law on housing and eviction issues within the HUDOC database.

Artificial intelligence and law·2026

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Accurate neurotransmitter detection is crucial for understanding biological functions.
  • Existing detection methods lack sufficient sensitivity and selectivity.
  • Monoamine neurotransmitters like dopamine (DA), norepinephrine (NE), and 3,4-dihydroxyphenylacetic acid (DOPAC) are key targets.

Purpose of the Study:

  • To develop a novel sensing mechanism for highly sensitive and selective neurotransmitter detection.
  • To investigate the potential of ultraviolet (UV) plasmonic engineered native fluorescence.
  • To evaluate the performance of aluminum hole array substrates for enhanced detection.

Main Methods:

  • Measurement of native fluorescence of DA, NE, and DOPAC.

More Related Videos

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

22.4K
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
09:10

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Published on: July 22, 2022

3.3K

Related Experiment Videos

Last Updated: Jul 11, 2025

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

13.2K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

22.4K
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
09:10

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Published on: July 22, 2022

3.3K
  • Utilizing aluminum hole array substrates with 300 nm hole spacing.
  • Analysis of fluorescence enhancement, photon yield, and photobleaching rates.
  • Proof-of-concept experiment involving mixtures of DA and NE.

    • Main Results:

    • Achieved average net enhancement of 50× and total photon yield enhancement of 60× for the studied neurotransmitters.
    • Observed a 1.5-1.7× reduction in photobleaching rate on aluminum hole arrays compared to thin films.
    • Demonstrated that photobleaching rates are sensitive to molecular structure and can be engineered by UV plasmonic substrates.
    • Showed a proportional relationship between NE concentration in mixtures and average photobleaching rate.

    Conclusions:

    • UV plasmonic engineered native fluorescence offers a promising approach for sensitive and selective neurotransmitter detection.
    • The engineered substrates significantly enhance fluorescence and modify photobleaching characteristics.
    • This method holds potential for advancing diagnostic and research tools in neuroscience and analytical chemistry.