[Variations in ultrastructures and negative charges in experimental hydrosalpinx]

It is not so difficult to operate on the hydrosalpinx with macrosurgical or microsurgical methods, but difficult to initiate pregnancy after the operation because little is known about the post-operative condition of the hydrosalpinx. In this study time sequential observations of the growing experimental hydrosalpinx with the two hemoclip method was performed during morphological change using the microscope, sequential electron microscope and transmission electron microscope with cationized ferritin as an ultrastructural marker. The following results were obtained: Size of hydrosalpinx: Until 15 weeks after the operation, the hemo-clipping hydrosalpinx became longer but thereafter there was no change. The maximum diameter of the hydrosalinx became wider until 20 weeks after treatment. Peristaltic of hydrosalpinx: Tubal peristaltics were observed until 7 weeks after treatment, but not thereafter. SEM findings: Decreases in the amount of cilia started 1 week after treatment, and 7 weeks after treatment partial excoriations of epithelium were observed. 20 weeks after treatment excoriations of epithelium were observed widely. A decrease in the amount of cilia was observed but no loss was observed. The negative charges on the tubal endometrium due to TEM using cationized ferritin: The negative charges on the tubal endometrium were decreasing both on the secretory cells and ciliary cells.