Related Experiment Video
Updated: Jul 11, 2025

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
A fatal misidentification: Burkholderia pseudomallei misidentified as Acinetobacter spp
Umang Agrawal1, Rasika Sirsat1, Anjali Shetty1
1PD Hinduja Hospital and Medical Research Centre, Veer Savarkar Marg, Mahim West, Mumbai, 400016, India.
None:
A middle-aged uncontrolled diabetic with chronic kidney disease presented with high-grade fever, skin abscesses and cough for two weeks. His blood cultures grew Burkholderia pseudomallei. A few weeks prior, blood cultures drawn for PUO workup elsewhere grew an organism identified as Acinetobacter sp with an unusual susceptibility pattern. His fever responded to a short course of meropenem. In retrospect, the earlier blood culture had likely misidentified B.pseudomallei as Acinetobacter sp given the background history, risk factors and the peculiar susceptibility report. Through this case, we discuss important aspects of melioid diagnostics which may be clinically relevant to establish this diagnosis.

