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Urinary miRNAs Predict Metastasis in Patients With Clinically Localized Clear Cell Renal Cell Carcinoma Treated With
Rodolfo Borges Dos Reis1, Xiang Shu2, Yuanqing Ye2
1Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirao Preto, Sao Paulo, Brazil.
Clinical Genitourinary Cancer
|November 9, 2023
Summary
Urine microRNAs (UmiRNAs) like miR-191-5p can predict metastasis risk in clear cell renal cell carcinoma (ccRCC) patients after surgery. Elevated pre-surgery UmiRNA levels indicate a higher chance of developing distant metastases.
Area of Science:
- Urology
- Oncology
- Molecular Biology
Background:
- Clear cell renal cell carcinoma (ccRCC) can metastasize post-surgery.
- Predictive biomarkers for metastasis are crucial for patient management.
Purpose of the Study:
- To investigate urine microRNA (UmiRNA) expression levels before and after nephrectomy in ccRCC patients.
- To determine the association between UmiRNA expression and the development of distant metastases.
Main Methods:
- Prospective collection of pre- and post-nephrectomy urine samples from 117 ccRCC patients.
- RT-PCR analysis to quantify 137 UmiRNAs.
- Cox regression modeling to assess the association between UmiRNA expression and metastasis development.
Main Results:
- Five UmiRNAs (miR-191-5p, miR-324-3p, miR-186-5p, miR-93-5p, miR-30b-5p) were upregulated pre-nephrectomy, increasing metastasis risk.
- miR-191-5p showed the strongest association with metastasis (HR = 4.16).
- miR-191-5p, miR-324-3p, and miR-186-5p were significantly associated with metastasis in pT3 tumors.
Conclusions:
- UmiR-191-5p, UmiR-324-3p, and UmiR-186-5p are potential biomarkers for predicting metastasis in ccRCC, especially in pT3 patients.
- UmiRNA expression changes may reflect tumor-specific alterations relevant for predicting metastasis in non-metastatic locally advanced ccRCC.
