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A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models.

Sebastian Junge1,2, Maria Elena Ricci Signorini3, Masa Al Masri1,2

  • 1Institute of Quantum Optics, Gottfried Wilhelm Leibniz University, 30167, Hannover, Germany.

Scientific Reports
|November 9, 2023
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Summary

This study introduces a micro-LED array for optogenetic control in cardiac research, enabling precise light stimulation of cardiac cells and tissues. The system effectively controls contraction and calcium waves in various cardiac models.

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Area of Science:

  • Biomedical Engineering
  • Cardiology
  • Optogenetics

Background:

  • Optogenetics requires precise light control for cardiac research.
  • Existing methods may lack flexibility in spatial and temporal light delivery.
  • Novel illumination systems are needed to advance cardiac optogenetic studies.

Purpose of the Study:

  • To develop and validate a compact micro-LED array system for optogenetic stimulation in cardiac research.
  • To demonstrate the system's capability in controlling cellular and tissue-level cardiac functions.
  • To assess the system's performance across different cardiac models and stimulation parameters.

Main Methods:

  • Integration of a 16x16 micro-LED array into a widefield fluorescence microscope.
  • Optical design ensuring full field-of-view coverage with various objectives.
  • Experiments using channelrhodopsin-2 expressing cardiomyocytes, cardiac bodies, and bioartificial cardiac tissues.
  • Characterization of pacing efficiency based on illumination parameters and stimulation frequency.
  • Demonstration of dynamic stimulation by steering calcium waves in HL-1 cell monolayers.

Main Results:

  • The micro-LED array system provides effective spatial and temporal light control.
  • Pacing efficiency was characterized across different cardiac models and stimulation parameters.
  • Dynamic steering of calcium waves was successfully achieved using patterned light.
  • The system demonstrated robust performance in controlling cardiac contraction and calcium dynamics.

Conclusions:

  • Micro-LED arrays are powerful tools for optogenetic control in cardiac research.
  • The developed system enables precise stimulation of cardiac monolayers, multicellular bodies, and 3D tissues.
  • This technology facilitates fundamental and therapeutic investigations in cardiac electrophysiology.