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Related Experiment Video

Updated: Jul 11, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Wide-field endoscope accessory for multiplexed fluorescence imaging.

Gaoming Li¹, Miki Lee¹, Tse-Shao Chang²

¹Division of Gastroenterology, Department of Internal Medicine, University of Michigan, 109 Zina Pitcher Pl. BSRB 1522, Ann Arbor, MI, 48109-2200, USA.

Scientific Reports

|November 9, 2023

Summary

A new flexible, fiber-coupled fluorescence endoscope accessory enables concurrent detection of multiple molecular targets. This technology allows for real-time, in vivo imaging, potentially improving the detection of diseases in hollow organs.

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Last Updated: Jul 11, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Area of Science:

Medical Endoscopy
Optical Imaging
Biomedical Engineering

Background:

Conventional white light endoscopy can be enhanced by fluorescence imaging for detecting multiple molecular targets simultaneously.
There is a need for flexible, fiber-coupled accessories compatible with standard medical endoscopes for clinical fluorescence imaging.

Purpose of the Study:

To demonstrate a flexible fiber-coupled accessory for standard medical endoscopes capable of collecting fluorescence images.
To evaluate the performance of this accessory for in vivo multiplexed fluorescence imaging.

Main Methods:

Designed and fabricated a miniature scan mirror (1.30 × 0.45 mm²) for wide angular deflections.
Integrated the scan mirror into a 2.6 mm diameter, 12 mm length distal tip, enabling real-time, wide field-of-view (FOV) image generation.
Collected multiplexed fluorescence images in vivo from mice using 3 fluorescently-labeled peptides at 10 frames per second.

Main Results:

Achieved maximum divergence angles of ±27.4° and ±22.8°, resulting in FOVs of 10.4 mm and 8.4 mm at a 10 mm working distance.
Successfully separated collected fluorescence images into 3 channels.
Measured target-to-background ratios of 2.6, 3.1, and 3.9.

Conclusions:

The developed flexible fiber-coupled fluorescence endoscope accessory is suitable for clinical use.
This technology shows potential for detecting heterogeneous diseases in hollow organs by imaging multiple molecular targets concurrently.