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Updated: Jul 11, 2025

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
Published on: October 3, 2010
Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
Amanda Faria Assoni1,2, Thiago Giove Mitsugi3, René Wardenaar4
1Human Genome and Stem Cell Research Center, Institute of Biosciences, University of São Paulo, 106, Rua do Matão, Cidade Universitária, São Paulo, 05508-090, Brazil. amandafassoni@gmail.com.
Abstract:
VAMP (Vesicle-associated membrane protein)-associated protein B and C (VAPB) has been widely studied in neurodegenerative diseases such as ALS, but little is known about its role in cancer. Medulloblastoma is a common brain malignancy in children and arises from undifferentiated cells during neuronal development. Therefore, medulloblastoma is an interesting model to investigate the possible relationship between VAPB and tumorigenesis. Here we demonstrate that high VAPB expression in medulloblastoma correlates with decreased overall patient survival. Consistent with this clinical correlation, we find that VAPB is required for normal proliferation rates of medulloblastoma cells in vitro and in vivo. Knockout of VAPB (VAPBKO) delayed cell cycle progression. Furthermore, transcript levels of WNT-related proteins were decreased in the VAPBKO. We conclude that VAPB is required for proliferation of medulloblastoma cells, thus revealing VAPB as a potential therapeutic target for medulloblastoma treatment.
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