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Antiproliferative effects of D-allose associated with reduced cell division frequency in glioblastoma
Kenta Suzuki1, Daisuke Ogawa1, Takahiro Kanda1
1Department of Neurological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
Abstract:
Recent studies have shown that D-allose, a rare sugar, elicits antitumor effects on different types of solid cancers, such as hepatocellular carcinoma, non-small-cell lung cancer, and squamous cell carcinoma of the head and neck. In this study, we examined the effects of D-allose on the proliferation of human glioblastoma (GBM) cell lines (i.e., U251MG and U87MG) in vitro and in vivo and the underlying mechanisms. D-allose treatment inhibited the proliferation of U251MG and U87MG cells in a dose-dependent manner (3-50 mM). However, D-allose treatment did not affect cell cycles or apoptosis in these cells but significantly decreased the cell division frequency in both GBM cell lines. In a subcutaneous U87MG cell xenograft model, intraperitoneal injection of D-allose (100 mg/kg/day) significantly reduced the tumor volume in 28 days. These data indicate that D-allose-induced reduction in cell proliferation is associated with a subsequent decrease in the number of cell divisions, independent of cell-cycle arrest and apoptosis. Thus, D-allose could be an attractive additive to therapeutic strategies for GBM.
Insights
D-allose, a rare sugar, effectively inhibits glioblastoma (GBM) cell proliferation and reduces tumor volume in vivo. This sugar impacts cell division frequency without affecting cell cycles or apoptosis, suggesting its potential in GBM therapy.
Area of Science:
- Oncology
- Biochemistry
Background:
- D-allose, a rare sugar, has demonstrated antitumor effects against various solid cancers.
- Glioblastoma (GBM) remains a challenging malignancy with limited effective therapeutic options.
Purpose of the Study:
- To investigate the anti-proliferative effects of D-allose on human glioblastoma (GBM) cell lines in vitro and in vivo.
- To elucidate the underlying mechanisms of D-allose's action on GBM cells.
Main Methods:
- In vitro proliferation assays using U251MG and U87MG GBM cell lines treated with varying concentrations of D-allose (3-50 mM).
- Cell cycle and apoptosis analyses were performed.
- In vivo studies utilizing a subcutaneous U87MG cell xenograft mouse model with daily intraperitoneal D-allose injections (100 mg/kg/day) over 28 days.
Main Results:
- D-allose significantly inhibited GBM cell proliferation in a dose-dependent manner.
- D-allose treatment did not alter cell cycle progression or induce apoptosis.
- A significant decrease in cell division frequency was observed in D-allose-treated GBM cells.
- In vivo, D-allose administration markedly reduced tumor volume in the xenograft model.
Conclusions:
- D-allose exhibits potent anti-proliferative effects on glioblastoma cells by reducing cell division frequency, independent of cell cycle arrest or apoptosis.
- These findings highlight D-allose as a promising candidate for novel therapeutic strategies against GBM.
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