Metabolic reactions responsible for glucose stimulation of alkaline phosphatase in Vibrio cholerae

Alkaline phosphatase activity in Vibrio cholerae strain 569B grown in low-phosphate medium was stimulated if glucose or glycerol was used as the carbon source. No such stimulation was observed, however, if tricarboxylic acid cycle intermediates like succinate or citrate were used. Experiments using specific enzyme inhibitors strongly indicated that the metabolic reactions of the glycolytic pathway from glyceraldehyde 3-phosphate to 2-phosphoglycerate play a key role in the stimulation process.