Related Experiment Videos
Analysis of hemangiopericytic meningiomas by immunohistochemistry, electron microscopy and cell culture
Abstract:
Intracranial hemangiopericytic meningiomas (HM) from seven patients were examined by immunostaining, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and cell culture. Positive staining for Factor VIII-related antigen was restricted to capillary endothelial cells. There was no reaction with anti-glial fibrillary acidic protein (GFAP) serum or anti-S-100 protein serum. In these neoplasms TEM displayed extracellular basement membrane-like material (BMLM), cytoplasmic intermediate filaments (IF) associated with a dense body, dilated rough-surfaced endoplasmic reticulum containing BMLM, a small area of interdigitation of cell membranes, and a unique intercellular punctate or linear density. By SEM these tumors had intercellular shell-like or reticular structures and irregularly branched capillaries which were compressed by ellipsoidal- or carrot-shaped tumor cells. Short-term monolayer culture showed rapid and vigorous growth of tumor cells, and the formation of pseudolumens but not of whorls. The TEM of cultured cells also showed cytoplasmic IF associated with the dense body. By SEM the cultured cells were flat and had a discoid nucleus with conspicuous nucleolar hillocks. Our results show that HM are mainly poorly specialized mesenchyme-related tumors of the meninges; some possess a potential for aggressive growth and some for differentiation into smooth muscle cells. Further study is needed to determine their histogenesis.
Insights
Intracranial hemangiopericytic meningiomas (HM) are mesenchyme-related tumors. These meningeal tumors show potential for aggressive growth and smooth muscle differentiation, requiring further histogenesis studies.
Area of Science:
- Neuro-oncology
- Tumor biology
- Cellular pathology
Background:
- Intracranial hemangiopericytic meningiomas (HM) are rare dural-based neoplasms.
- Understanding their cellular origin and behavior is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the ultrastructural and cellular characteristics of intracranial hemangiopericytic meningiomas.
- To elucidate the potential for growth and differentiation of these tumors.
Main Methods:
- Immunostaining for Factor VIII-related antigen, GFAP, and S-100 protein.
- Transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
- Short-term monolayer cell culture.
Main Results:
- Immunostaining confirmed Factor VIII-related antigen in capillary endothelial cells, with no GFAP or S-100 protein expression.
- TEM revealed extracellular basement membrane-like material, intermediate filaments, and unique intercellular densities.
- SEM showed intercellular structures and compressed capillaries; cell culture demonstrated rapid growth and pseudolumen formation.
Conclusions:
- Intracranial hemangiopericytic meningiomas are primarily poorly specialized, mesenchyme-related meningeal tumors.
- These tumors exhibit potential for aggressive growth and differentiation into smooth muscle cells.
- Further research is necessary to fully determine the histogenesis of HM.