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Related Concept Videos

Positron Emission Tomography01:29

Positron Emission Tomography

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Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
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Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

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Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
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Related Experiment Video

Updated: Jul 11, 2025

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PET/NIR Fluorescence Bimodal Imaging for Targeted Tumor Detection.

Xin Xue1, Qiyi Li2, Pengjun Zhang3

  • 1Department of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu China.

Molecular Pharmaceutics
|November 10, 2023
PubMed
Summary

A novel bimodal probe, [68Ga]Ga-DOTA-NPC, enhances colon cancer detection and surgical guidance. This imaging agent combines positron emission tomography (PET) and near-infrared fluorescence (NIRF) for improved tumor visualization and resection.

Keywords:
NIR fluorescence imagingPETbimodal imaging probeimage-guided surgerytumor targeting

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Area of Science:

  • Oncology
  • Medical Imaging
  • Radiochemistry

Background:

  • Cancer diagnosis and treatment are challenged by late detection and incomplete tumor removal.
  • Bimodal probes integrating positron emission tomography (PET) and near-infrared fluorescence (NIRF) offer potential for comprehensive cancer management.
  • Targeting specific cancer cell mechanisms, like organic anion-transporting polypeptide 1B3 (OATP1B3), can improve imaging agent specificity.

Purpose of the Study:

  • To develop and evaluate a novel PET/NIRF bimodal imaging agent, [68Ga]Ga-DOTA-NPC, for colon cancer detection and surgical guidance.
  • To assess the probe's specificity for colon cancer cells expressing OATP1B3.
  • To investigate the in vitro and in vivo performance of [68Ga]Ga-DOTA-NPC for tumor imaging and resection.

Main Methods:

  • Synthesis of the PET/NIRF bimodal probe [68Ga]Ga-DOTA-NPC by coupling DCDSTCY and DOTA via ethylenediamine and radiolabeling with gallium-68.
  • In vitro evaluation of probe uptake in colon cancer cells.
  • In vivo studies using orthotopic and subcutaneous colon tumor models in animals for PET/NIRF imaging and surgical guidance.

Main Results:

  • The probe [68Ga]Ga-DOTA-NPC demonstrated preferential uptake in colon cancer cells, attributed to OATP1B3 expression.
  • PET/NIRF imaging confirmed high-contrast tumor accumulation and good biosafety of the probe.
  • Successful resection of orthotopic and subcutaneous colon tumors was achieved under real-time NIRF guidance.

Conclusions:

  • [68Ga]Ga-DOTA-NPC is a promising bimodal imaging agent for sensitive, high-contrast detection of colon cancer.
  • The probe facilitates real-time intraoperative surgical guidance for complete tumor resection.
  • [68Ga]Ga-DOTA-NPC offers advanced PET/NIRF imaging capabilities for differentiating tumors from surrounding tissues.