Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic disorder is crucial for mitochondrial protein import.

Protein science : a publication of the Protein Society·2026
Same author

Inactivation of cofilin-1 in Mcpt5-Cre-nf-Cfl1<sup>fl/fl</sup> mice prevents the formation of connective tissue mast cells without affecting basophils: a new tool to investigate the specific role of CTMCs in disease.

Frontiers in immunology·2026
Same author

A dynamic displacement mechanism drives protein import into mitochondria.

bioRxiv : the preprint server for biology·2026
Same author

A Novel HMBC-CC-HMQC NMR Strategy for Methyl Assignment Using Triple-<sup>13</sup>C-Labeled α-Ketoisovalerate Integrated with UCBShift 2.0.

Journal of molecular biology·2025
Same author

Integrin beta 1 facilitates non-enveloped hepatitis E virus cell entry through the recycling endosome.

Nature communications·2025
Same author

Reconstitution of interferon regulatory factor 7 expression restores interferon beta induction in Huh7 cells.

Journal of virology·2025

Related Experiment Video

Updated: Jul 11, 2025

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.2K

Cellular ROS tolerance determines the effect of plumbagin on osteoclast differentiation.

Sevinj Sultanli1,2, Jakob Schneider3, Sandy S Burkart3

  • 1Heidelberg University, Medical Faculty Heidelberg, Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg, Germany.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|November 11, 2023
PubMed
Summary

Plumbagin induces reactive oxygen species (ROS) and apoptosis in RAW 264.7 cells, hindering osteoclast formation. This highlights the importance of cell-specific responses when studying phytochemical effects.

Keywords:
ROSapoptosiscancerglutathioneosteoclastphytochemicalplumbagin

More Related Videos

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.2K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.7K

Related Experiment Videos

Last Updated: Jul 11, 2025

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.2K
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.2K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Plumbagin, a naphthoquinone, possesses anti-inflammatory and anti-microbial properties.
  • Plumbagin induces reactive oxygen species (ROS) and apoptosis in cancer cells.
  • Cancer-related inflammation can impact bone density, suggesting plumbagin's potential to inhibit osteoclast formation.

Purpose of the Study:

  • To investigate the differential effects of plumbagin on osteoclastogenesis in primary macrophages versus the RAW 264.7 cell line.
  • To elucidate the role of ROS in plumbagin's impact on osteoclast differentiation.
  • To understand cell-intrinsic properties influencing responses to plumbagin.

Main Methods:

  • Comparing plumbagin's effects on osteoclastogenesis in primary bone marrow-derived macrophages and RAW 264.7 cells.
  • Assessing ROS production and cell viability in response to plumbagin and RANKL.
  • Utilizing N-acetyl cysteine as a ROS scavenger to evaluate its protective effects.
  • Analyzing gene expression related to oxidative stress resistance.

Main Results:

  • RAW 264.7 cells are more susceptible to plumbagin-induced apoptosis than primary macrophages.
  • Plumbagin, in combination with RANKL, increases ROS production in RAW 264.7 cells, leading to cell death.
  • N-acetyl cysteine treatment rescues RAW 264.7 cells from plumbagin-induced death, confirming ROS involvement.
  • RAW 264.7 cells exhibit lower expression of oxidative stress-protective genes compared to primary macrophages.

Conclusions:

  • Cell-intrinsic properties significantly influence the response to plumbagin, particularly regarding ROS tolerance and apoptosis.
  • The differential sensitivity of cell types must be considered when evaluating plumbagin's therapeutic potential, especially concerning bone metabolism.
  • Plumbagin's ability to inhibit osteoclastogenesis is linked to its capacity to induce ROS and trigger apoptosis in a cell-dependent manner.