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Published on: April 22, 2016
Immobilized Cellulase on NH2-MIL-88(Fe) and Its Performance as a Biocatalyst
Jing Jiang1, Xiaowu Gong2, Tiantian Li1
1School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green, Processing of Chemical Engineering, Shihezi University, Shihezi, 832003, China.
Abstract:
To broaden pH range and improve thermal stability, reusability, storage stability, and organic solvent tolerance of natural enzymes, a magnetic material (NH2-MIL-88(Fe)) was synthesized as a new material to immobilize cellulase. The results showed that the optimal temperature and pH of cellulase immobilized on NH2-MIL-88(Fe) showed a wider range compared to free cellulase, and 74% and 83% of the initial activity could be retained after 10 cycles and storage for 49 days, respectively. Moreover, the tolerance for organic solvents was improved compared with free enzyme. The reducing sugar yields from sodium carboxymethylcellulose (CMC) and corn cob hydrolyzed with cellulase immobilized on NH2-MIL-88(Fe) were higher than observed with the free enzyme, which demonstrated the better biocatalytic performance of cellulase immobilized on NH2-MIL-88(Fe).
