Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

29
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
29
Hydrolysis01:15

Hydrolysis

105.5K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
105.5K
Amino Acid Catabolism01:18

Amino Acid Catabolism

28
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
28
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of machine learning-based model for macrosomia and spontaneous preterm birth: A retrospective cohort study.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2026
Same author

Engineering Escherichia coli BL21(DE3) for efficient production of D-allulose from D-glucose via phosphorylation/epimerization/dephosphorylation pathway.

Enzyme and microbial technology·2026
Same author

ADA-YOLO: An Adaptive Dynamic Aggregation Network for Small Object Detection in UAV Imagery.

Sensors (Basel, Switzerland)·2026
Same author

Biochemical Characterization of a Novel Galactitol 2-Dehydrogenase from a <i>Ciceribacter</i> sp. L1K22 with High Catalytic Efficiency for d-tagatose Production.

Journal of agricultural and food chemistry·2026
Same author

Biosynthesis of Galactooligosaccharides: Enzymatic Strategies, Physiological Functions, and Applications.

Journal of agricultural and food chemistry·2026
Same author

A sensitive UHPLC-QqQ-MS/MS method for the simultaneous analysis of MDA/4-HHE/4-HNE in edible oils and nuts was developed by derivatization optimization and SPE purification.

Food chemistry·2026
Same journal

Enhancing flavor quality in low-salt dried large yellow croaker (Pseudosciaena crocea) through secondary fermentation: insights into microbial drivers.

Food chemistry·2026
Same journal

Comparative analysis of appearance, digestive behaviour, and nutritional quality of grilled alternative meat analogues and beef patty.

Food chemistry·2026
Same journal

Fabrication and characterization of sunflower oil body-κ-carrageenan emulsion gels: Gelation behavior, rheological properties, and interaction mechanisms.

Food chemistry·2026
Same journal

Complexation between mannoprotein-grape polysaccharide nanoparticles and quercetin was associated with color restoration: A mechanistic exploration in model red wine solutions.

Food chemistry·2026
Same journal

Phenolics in sesame meal protein: Structural characterization, interaction mechanism, and impacts on protein functional properties.

Food chemistry·2026
Same journal

Gastrointestinal digestive fate of squid cartilage collagen peptides: degradation characteristics and the key role of Gly-Pro skeleton structure.

Food chemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 11, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.4K

Microbial dextran-hydrolyzing enzyme: Properties, structural features, and versatile applications.

Ziwei Chen1, Jiajun Chen2, Dawei Ni2

  • 1School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.

Food Chemistry
|November 11, 2023
PubMed
Summary
This summary is machine-generated.

Dextranase enzymes, found in various microbes, break down dextran into useful compounds for food and medicine. Understanding their structure and function is key to unlocking new industrial applications.

Keywords:
ApplicationsDextranaseEnzymatic propertiesOligodextransStructures

More Related Videos

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
06:16

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

20.6K

Related Experiment Videos

Last Updated: Jul 11, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.4K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
06:16

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

20.6K

Area of Science:

  • Enzymology
  • Biotechnology
  • Carbohydrate Chemistry

Background:

  • Dextran, an alpha-glucan with (α1→6) linkages, is widely used in food, cosmetics, and medicine.
  • Dextranase hydrolyzes dextran, producing oligodextrans with significant food industry applications.
  • Dextranases are microbial enzymes classified into glycoside hydrolase (GH) families 13, 15, 31, 49, and 66.

Purpose of the Study:

  • To review the enzymatic properties, structural features, and applications of dextranase.
  • To highlight the potential of dextranase in industries like sugar production, oral care, medicine, and biotechnology.
  • To discuss the classification, microbial distribution, and immobilization of dextranase.

Main Methods:

  • Literature review of existing research on dextranase.
  • Analysis of solved crystal structures of dextranases from GH families 13, 15, 31, 49, and 66.
  • Discussion of dextranase classification, microbial sources, and immobilization techniques.

Main Results:

  • Seven crystal structures of dextranases have been determined.
  • The molecular mechanisms of dextran hydrolysis by dextranases are not fully understood.
  • Dextranase exhibits broad potential across various industrial sectors.

Conclusions:

  • Dextranase is a versatile enzyme with significant industrial potential.
  • Further research into dextranase's molecular mechanisms is needed.
  • This review provides a foundation for future advancements in dextranase technology.