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Protease detection in the biosensor era: A review
Pratika Rai1, Sabrina N Hoba2, Celine Buchmann1
1Faculty of Computer Sciences and Microsystems Technology, Kaiserslautern University of Applied Sciences, Amerikastr.1, 66482, Zweibrücken, Germany.
Biosensors & Bioelectronics
|November 12, 2023
Summary
Protease biosensors offer efficient detection of disease biomarkers. This review compares optical and electrochemical methods for designing versatile protease biosensors, aiding research and point-of-care applications.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biochemistry
Background:
- Proteases are implicated as biomarkers in diseases like cancer and cardiovascular conditions.
- Protease dysregulation affects extracellular matrix and basement membrane.
- Traditional detection assays lack efficiency compared to modern biosensors.
Purpose of the Study:
- To review optical and electrochemical detection methods for protease biosensors.
- To analyze and compare transducer techniques for protease biosensing platforms.
- To discuss the impact of nanomaterials on biosensor performance.
Main Methods:
- Review of existing literature on protease biosensors.
- Analysis of activity-based biosensors utilizing peptide biorecognition elements.
- Comparison of various transducer techniques (optical and electrochemical).
Main Results:
- Optical and electrochemical methods offer efficient protease detection.
- Activity-based biosensors with peptide elements are a key focus.
- Nanomaterials can enhance biosensor performance.
Conclusions:
- This review aids in selecting appropriate biosensor designs for protease detection.
- Protease biosensors show promise for improved disease understanding and point-of-care testing.
- Further development of protease biosensors can advance diagnostic capabilities.
