Redox reactions in vitrified-warmed ovary
Atefe Rahimi1, Ali Shahriari1, Farid Barati2
1Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
F&S Science
|November 12, 2023
Summary
Ovary vitrification significantly disrupts the oxidant/antioxidant balance, decreasing total antioxidant capacity and superoxide dismutase while increasing malondialdehyde. These findings highlight oxidative stress challenges in cryopreservation.
Area of Science:
- Reproductive biology
- Cryobiology
- Biochemistry
Background:
- Ovary vitrification is crucial for fertility preservation and species conservation.
- Cryopreservation of complex tissues like ovaries faces challenges, notably oxidative stress damage.
Purpose of the Study:
- To assess oxidative stress markers in vitrified bovine ovaries.
- To understand the impact of vitrification on ovarian tissue antioxidant status.
Main Methods:
- Bovine ovarian cortex pieces were vitrified using ethylene glycol, glycerol, and sucrose.
- Warming involved descending sucrose concentrations.
- Biochemical assays were performed to measure oxidative stress indicators.
Main Results:
- Vitrification led to a 45% decrease in total antioxidant capacity (P<.0001).
- Malondialdehyde increased fourfold (P=.0002), and superoxide dismutase decreased by 53% (P=.0081).
- Protein carbonyl levels were lower in vitrified-warmed ovaries compared to fresh ones (P=.0325).
Conclusions:
- Ovary vitrification causes significant alterations in the oxidant/antioxidant balance.
- The study reveals distinct oxidative stress profiles in vitrified-warmed ovarian tissues compared to fresh tissues.
