Dual-mode sensing chip for photoelectrochemical and electrochromic visual determination of deoxynivalenol mycotoxin

The successful development of a dual-mode sensing chip for deoxynivalenol (DON) detection using photoelectrochemical (PEC) and electrochromic visualization techniques is reported. By laser etching technology, different functional areas, including the photoanode, the cathode, and the electrochromic area, are fabricated on indium tin oxide (ITO) glass. Then, these three areas are further respectively modified with PEC active materials, platinum nanoparticles, and Prussian blue. Under light illumination, photocurrents generate between the photoanode and the cathode due to the separation of photo-induced electrons and holes in the TiO₂/3DNGH material. Meanwhile, the photo-induced electrons are transferred to Prussian blue on the visualization area, which will be reduced to colorless Prussian white. The binding of DON molecules and aptamers can promote electron transfer and reduce the recombination of electrons and holes, allowing for simultaneous quantitative detection of DON using either the photocurrent or color change. The sensor chip has a broad detection range of DON concentrations of 1 fg⋅mL^-1 to 100 pg⋅mL^-1 in the PEC mode with the limit of detection of 0.37 fg⋅mL^-1, and 1 to 250 ng⋅mL^-1 in the visualization mode with the limit of detection of 0.51 ng⋅mL^-1. This portable dual-mode sensor chip can be used in both laboratory and field settings without the need for specialized instruments, making it a powerful tool for ensuring food safety. At the same time, the analysis of the standard addition method of the actual sample by using the sensor chip shows that, in the PEC mode, the recoveries of the dual-mode aptasensor chip were 91.3 to 99.0% with RSD values of 1.73~2.55%, and in visualization mode, the recoveries of the dual-mode aptasensor chip were 99.2 to 102.0% with RSD values of 1.00~6.21%, which indicate good accuracy and reproducibility.