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Updated: Jul 11, 2025

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Thymidine kinase 1 as a target is regulated by the hsa-let-7b-5p/LINC00665 axis and affects NSCLC prognosis

Xu-Dong Zhu1, Yong-Fei Fan1, Yi Zhao1

  • 1Department of Thoracic Surgery, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, 213003, China.

Heliyon
|November 13, 2023
PubMed
Abstract

Insights

High thymidine kinase 1 (TK1) expression in non-small cell lung cancer (NSCLC) correlates with worse prognosis. The LINC00665/has-let-7b-5p/TK1 network may serve as a prognostic biomarker and therapeutic target for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Competitive endogenous RNA (ceRNA) networks are implicated in tumor growth and represent potential therapeutic targets.
  • Thymidine kinase 1 (TK1) is increasingly recognized for its role in cancer development.

Purpose of the Study:

  • To investigate the impact of TK1-related ceRNA networks on the prognosis of non-small cell lung cancer (NSCLC).
  • To identify potential prognostic biomarkers and therapeutic targets for NSCLC based on TK1 expression and its regulatory network.

Main Methods:

  • Retrieved TK1 expression data from The Cancer Genome Atlas (TCGA) for NSCLC and normal tissues.
  • Utilized immunohistochemistry and clinical follow-up data for prognostic analysis.
  • Employed the starBase database to identify TK1-targeting microRNAs and long non-coding RNAs to construct a ceRNA network.

Main Results:

  • Significantly higher TK1 expression was observed in NSCLC tissues compared to normal tissues.
  • Elevated TK1 expression correlated with a poorer prognosis in NSCLC patients.
  • The LINC00665/has-let-7b-5p/TK1 network was identified as a key regulator influencing NSCLC growth and prognosis.
  • TK1 methylation and immune cell infiltration were associated with NSCLC prognosis.

Conclusions:

  • Elevated TK1 levels, potentially mediated by the LINC00665/has-let-7b-5p/TK1 network, significantly impact NSCLC prognosis.
  • TK1 serves as a potential prognostic biomarker and therapeutic target for NSCLC.
  • Further research into TK1-related ceRNA networks could lead to novel treatment strategies for NSCLC.
Keywords:
LINC00665NSCLCTK1ceRNAlet-7b-5p

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