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A novel copper-based nanozyme: fabrication and application for colorimetric detection of resveratrol
Wanxin Li1, Jiaxin Sun1, Yao Xin1
1Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130023, P. R. China. wangzhi@jlu.edu.cn.
Abstract:
A novel nanozyme (urea@Cu-NF) was synthesized by self-assembly of urea and copper phosphate with urea as plasticizer. Urea@Cu-NF exhibited excellent peroxidase-like activity with the ability to oxidize TMB in the presence of H2O2. However, its peroxidase-like activity could be inhibited by resveratrol, leading to an absorption decrease in the intensity of oxTMB. Based on this phenomenon, a colorimetric method was designed for resveratrol detection. The colorimetric reaction could be completed within 20 min with a linear range of 1-120 μM. The limit of detection (LOD) of resveratrol is 0.43 μM. Our experimental results demonstrate that urea@Cu-NF has enormous potential to function as a cheap and accurate quality detection tool.
