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Modular 3D printed platform for fluidically connected human brain organoid culture
Babak Rezaei1, Jessica Giacomoni2, Fredrik Nilsson2
1National Centre for Nano Fabrication and Characterization (DTU Nanolab), Technical University of Denmark, Kgs. Lyngby, Denmark.
Biofabrication
|November 13, 2023
Summary
Researchers developed a 3D printing pipeline for custom brain organoid culture platforms. This innovation streamlines the entire process, reducing plastic waste and improving culturing conditions for brain organoids.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Biotechnology
Background:
- Brain organoid technology offers novel insights into human brain development and diseases.
- Current brain organoid protocols often use suboptimal, non-specialized culturing tools, leading to inefficiencies and plastic waste.
- There is a need for advanced bioengineering solutions to support the growing use of brain organoids.
Purpose of the Study:
- To develop a 3D printing pipeline for fabricating customized, multi-well culturing platforms for brain organoids.
- To create an integrated system enabling all brain organoid culture steps within a single platform, minimizing manipulation.
- To provide a modular and adaptable solution using accessible materials and 3D printing technology.
Main Methods:
- Fabrication of tailor-made culturing platforms using a 3D printing pipeline.
- Design of fluidically connected yet spatially separated brain organoid arrays.
- Implementation of an all-in-one platform for cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation.
- Utilization of accessible materials and standard 3D printing equipment.
Main Results:
- Successful development of a 3D printable culturing platform for brain organoid arrays.
- Demonstration of an all-in-one workflow within a single well plate, eliminating the need for organoid transfer.
- The platform supports all stages of brain organoid culture, from initial aggregation to maturation.
- The approach utilizes accessible materials and widely available 3D printing technology.
Conclusions:
- The developed 3D printing pipeline provides a customizable and efficient solution for brain organoid culture.
- This technology addresses the limitations of existing culturing methods, improving conditions and reducing plastic usage.
- The modular design allows for adaptation by other research groups, potentially accelerating advancements in brain organoid research and other 3D cell culture systems.
