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Evaluating Tartary Buckwheat Genotypes with High Callus Induction Rates and the Transcriptomic Profiling during
Haixia Zhao1, Xin Li1, Xin Xiao1
1College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Plants (Basel, Switzerland)
|November 14, 2023
Summary
Researchers identified key genes and pathways involved in Tartary buckwheat callus formation. This study enhances understanding of Tartary buckwheat
Area of Science:
- Plant Molecular Biology
- Agricultural Biotechnology
Background:
- Tartary buckwheat (TB) research is limited by complex genetics and low regeneration rates.
- Developing efficient methods for TB molecular breeding and biological studies is crucial.
Purpose of the Study:
- To investigate the molecular mechanisms underlying callus formation (CF) in Tartary buckwheat.
- To identify key genes and pathways regulating CF for improved breeding.
Main Methods:
- Hypocotyl explants from 60 TB genotypes were used, with TBC14 selected for high callus induction.
- Transcriptome sequencing and analysis of calli at different developmental stages.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed.
Main Results:
- DNA binding transcription factor activity was the most significant gene set identified.
- Enriched pathways included metabolism, secondary metabolite biosynthesis, and hormone signal transduction.
- Key genes identified include MYB, AP2/ERF, bHLH TFs, auxin/cytokinin-related genes, and FtPinG0008123200.01 (bZIP).
Conclusions:
- This study provides valuable insights into the molecular basis of Tartary buckwheat callus formation.
- New candidate genes regulating CF were identified, offering potential for molecular breeding applications.
