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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Dual-Lifetime Referencing (t-DLR) Optical Fiber Fluorescent pH Sensor for Microenvironments.

Wan-Har Chen¹, Evelyn Armstrong², Peter W Dillingham^3,4

¹Department of Chemistry, University of Otago, Dunedin 9054, New Zealand.

Sensors (Basel, Switzerland)

|November 14, 2023

Summary

A new optical fiber pH sensor measures microenvironment pH near aquatic species. This technology helps understand ocean acidification impacts on marine life.

Keywords:

dual-layer sensing film inverse calibration marine microenvironments ocean acidification optical fiber fluorescent pH sensor time-domain dual-lifetime referencing

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Area of Science:

Marine Biology
Oceanography
Sensor Technology

Background:

The diffusion boundary layer (DBL) microenvironment around aquatic organisms exhibits unique pH dynamics.
DBL pH differs from bulk seawater, potentially mitigating ocean acidification effects for calcifying species.

Purpose of the Study:

To develop a low-cost optical fiber fluorescent pH sensor for measuring pH in the DBL.
To assess the sensor's performance and suitability for real-time monitoring of metabolic-induced pH changes.

Main Methods:

A time-domain dual-lifetime referencing (t-DLR) system was coupled with a dual-layer sol-gel coated optical fiber pH sensor.
The sensor employed pH-sensitive iminocoumarin and pH-insensitive Ru(dpp)3-PAN, with measurements validated against GOA-ON guidelines.
Real-time pH measurements were conducted in the DBL of Ulva sp. seaweed.

Main Results:

The developed sensor demonstrated a dynamic pH range of 7.41-9.42, response time of 29-100 s, and minimal salinity dependency.
Sensor precision was ~0.02 pH units, meeting GOA-ON 'weather' guidelines.
Successful real-time pH monitoring in the seaweed DBL confirmed its practicability.

Conclusions:

The developed t-DLR optical fiber pH sensor is a practical tool for DBL microenvironment studies.
This technology can provide crucial insights into organismal responses to changing ocean conditions.
The sensor facilitates understanding of light-controlled metabolic activities influencing aquatic pH.