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Related Experiment Videos

Autofluorescence and ageing: changes in ribosome accuracy and lysosome [corrected] function.

J H Buchanan, J Sidhu

    Mechanisms of Ageing and Development
    |November 14, 1986
    PubMed
    Summary

    Autofluorescence in fibroblast cultures increases with reduced cell division and exposure to lysosome-accumulating compounds like aminoglycosides. This suggests impaired lysosome function may disrupt cellular homeostasis.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Toxicology

    Background:

    • Cellular autofluorescence is an indicator of cellular health.
    • Lysosomes play a critical role in cellular waste disposal and homeostasis.
    • Aminoglycosides are known to accumulate in lysosomes and can affect protein synthesis.

    Purpose of the Study:

    • To investigate the relationship between cellular autofluorescence, proliferative capacity, and lysosomal function.
    • To explore the impact of specific aminoglycosides and ammonium chloride on fibroblast autofluorescence and proliferation.

    Main Methods:

    • Culturing fibroblasts and measuring autofluorescence.
    • Treating cell cultures with streptomycin, paromomycin, G418, and ammonium chloride.
    • Assessing the effect of these compounds on cell proliferation and lysosomal function.

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    Main Results:

    • Autofluorescence intensity correlated inversely with the proliferative capacity of fibroblast cultures.
    • Treatment with aminoglycosides (streptomycin, paromomycin, G418) and ammonium chloride significantly increased autofluorescence.
    • Paromomycin and G418 demonstrated a reduction in cell proliferative capacity.

    Conclusions:

    • Aminoglycosides and ammonium chloride, by accumulating in lysosomes, may lead to a loss of homeostasis between protein synthesis errors and lysosome function.
    • Increased autofluorescence is likely a consequence of impaired lysosome function, potentially due to dysregulation of intralysosomal pH.