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Autofluorescence and ageing: changes in ribosome accuracy and lysosome [corrected] function
Mechanisms of Ageing and Development
|November 14, 1986
Summary
Autofluorescence in fibroblast cultures increases with reduced cell division and exposure to lysosome-accumulating compounds like aminoglycosides. This suggests impaired lysosome function may disrupt cellular homeostasis.
Area of Science:
- Cell Biology
- Biochemistry
- Toxicology
Background:
- Cellular autofluorescence is an indicator of cellular health.
- Lysosomes play a critical role in cellular waste disposal and homeostasis.
- Aminoglycosides are known to accumulate in lysosomes and can affect protein synthesis.
Purpose of the Study:
- To investigate the relationship between cellular autofluorescence, proliferative capacity, and lysosomal function.
- To explore the impact of specific aminoglycosides and ammonium chloride on fibroblast autofluorescence and proliferation.
Main Methods:
- Culturing fibroblasts and measuring autofluorescence.
- Treating cell cultures with streptomycin, paromomycin, G418, and ammonium chloride.
- Assessing the effect of these compounds on cell proliferation and lysosomal function.
Main Results:
- Autofluorescence intensity correlated inversely with the proliferative capacity of fibroblast cultures.
- Treatment with aminoglycosides (streptomycin, paromomycin, G418) and ammonium chloride significantly increased autofluorescence.
- Paromomycin and G418 demonstrated a reduction in cell proliferative capacity.
Conclusions:
- Aminoglycosides and ammonium chloride, by accumulating in lysosomes, may lead to a loss of homeostasis between protein synthesis errors and lysosome function.
- Increased autofluorescence is likely a consequence of impaired lysosome function, potentially due to dysregulation of intralysosomal pH.