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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

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Updated: Jun 23, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Expression of Lanthipeptides in Human Cells.

Sara M Eslami¹, Imran R Rahman², Wilfred A van der Donk^1,2

¹Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Biorxiv : the Preprint Server for Biology

|November 14, 2023

Summary

Cyclic peptides, like lanthipeptides, offer therapeutic potential due to their stability. This study develops a system to produce and target these peptides in mammalian cells for drug discovery.

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Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Cyclic peptides, including ribosomally synthesized and post-translationally modified peptides (RiPPs), are conformationally constrained and protease-resistant.
Lanthipeptide RiPP cytolysin L possesses a unique covalently stabilized helical structure.
This structure holds potential for disrupting protein-protein interactions.

Approach:

Developed an expression system for producing lanthipeptides and cytolysin L derivatives in mammalian cells.
Demonstrated successful nuclear targeting of these lanthipeptides.
Established a method for in vivo screening of lanthipeptide inhibitors.

Key Points:

Mammalian cell expression system for lanthipeptide production.
Demonstrated nuclear localization of produced lanthipeptides.
Potential for lanthipeptides to inhibit protein-protein interactions.

Conclusions:

Lanthipeptide production and nuclear targeting achieved in mammalian cells.
This system enables screening for lanthipeptide inhibitors of protein-protein interactions.
Opens avenues for therapeutic applications of cyclic peptides.