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Updated: Aug 13, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
Phenobarbital competes with diacylglycerol for protein kinase C
Abstract:
Phenobarbital inhibits protein kinase C of rat brain by competitively displacing the effector of the enzyme, diacylglycerol. The drug appears to occupy the triple hydrogen bonding site which bonds diacylglycerol - and also phorbol esters - to the enzyme. It remains to be seen if the effect is responsible for the pharmaceutical activity of the drug; even so, it provides an example of a restructuring of lipid-protein hydrogen bonding, in the hydrogen belt of the membrane, in a manner postulated as a mechanism of anesthesia.
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