Jove
Visualize
Contact Us

Related Concept Videos

X-Inactivation01:58

X-Inactivation

38.9K
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
38.9K
Leaky Scanning02:28

Leaky Scanning

4.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
4.5K
Leveling Effect01:29

Leveling Effect

1.6K
In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the...
1.6K
Natural Selection and Adaptation01:15

Natural Selection and Adaptation

1.8K
Natural selection, a fundamental concept in evolutionary biology, is the mechanism by which evolution is driven, favoring organisms that are best adapted to their environments. This process enhances their chances of survival and reproduction. Adaptation, a key outcome of this process, involves genetic modifications that optimize an organism's functionality under specific environmental challenges, such as extreme cold or thinner air at high altitudes.
Beyond physical adaptations,...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tailoring amine-rich porous monolith for the miniaturized solid phase extraction of phenolic environmental estrogens.

Talanta·2026
Same author

From food-medicine homology to clinical potential: Plant-derived vesicle-like nanoparticles for inflammation management.

Pharmacological research·2026
Same author

Symmetry classification of magnetic orders using oriented spin space groups.

Nature·2026
Same author

Xeno-nucleic acids support formation of Ag(I)-mediated duplexes and silver nanoclusters.

Nucleic acids research·2026
Same author

Allele-specific knockdown by an engineered DNAzyme capable of RNase H1 evasion.

Nucleic acids research·2026
Same author

Rapid evolution of a highly efficient RNA polymerase by homologous recombination.

Nature chemical biology·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

24.9K

Functionally Enhanced XNA Aptamers Discovered by Parallelized Library Screening.

Adriana Lozoya-Colinas1, Yutong Yu1, John C Chaput1,2,3,4

  • 1Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California 92697-3958, United States.

Journal of the American Chemical Society
|November 14, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel single-round screening method for discovering nucleic acid aptamers. This approach accelerates the identification of high-affinity aptamers for therapeutic targets, including SARS-CoV-2 proteins.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.3K
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.7K

Related Experiment Videos

Last Updated: May 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

24.9K
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.3K
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Nucleic acid aptamers are crucial for targeting disease-associated proteins.
  • Conventional in vitro evolution methods (SELEX) are time-consuming and limit exploration of sequence and target combinations.

Purpose of the Study:

  • To develop a rapid, single-round screening method for aptamer discovery.
  • To accelerate the identification of high-affinity aptamers against therapeutic targets.

Main Methods:

  • A single-round screening approach utilizing function-enhancing chemotypes.
  • Affinity selection of threomers against the SARS-CoV-2 S1 protein receptor binding domain.
  • Biochemical characterization of selected aptamers.

Main Results:

  • Demonstrated de novo discovery of aptamers using the new method.
  • Identified threomers with binding affinities comparable to conventional SELEX-generated aptamers.
  • Established a highly parallelizable method for aptamer discovery.

Conclusions:

  • The single-round screening approach significantly accelerates aptamer discovery.
  • This method offers a viable route for discovering therapeutic aptamers more efficiently.
  • The approach enables parallel exploration of diverse chemical repertoires for drug development.