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Updated: Jul 11, 2025

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Triblock Proteins with Weakly Dimerizing Terminal Blocks and an Intrinsically Disordered Region for Rational Design

Dmitrii Fedorov1,2, Nelmary Roas-Escalona1,2, Dmitry Tolmachev2,3

  • 1Department of Bioproducts and Biosystems, Aalto University, P.O. Box 16100, Aalto, FI-00076, Finland.

Small (Weinheim an Der Bergstrasse, Germany)
|November 15, 2023
PubMed
Summary

Researchers engineered protein triblocks to control liquid-liquid phase separation for creating functional condensates. Weak interactions in terminal blocks predictably tune condensate formation, enabling applications in synthetic biology and materials science.

Keywords:
biomaterialscoacervateliquid–liquid phase separationprotein dimerprotein engineering

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