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Updated: Jul 11, 2025
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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
Published on: September 10, 2020
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Structure-function and engineering of plant UDP-glycosyltransferase
Mengya Wang1,2, Qiushuang Ji1,2, Bin Lai3
1Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Computational and Structural Biotechnology Journal
|November 15, 2023
Summary
This review details plant uridine diphosphate-dependent glycosyltransferases (UGTs), crucial enzymes in natural product biosynthesis. It covers their structures, mechanisms, and protein engineering strategies for enhanced activity.
Area of Science:
- Biochemistry
- Plant Science
- Enzymology
Background:
- Natural products from plants hold significant industrial and medicinal value.
- Uridine diphosphate-dependent glycosyltransferases (UGTs) are key enzymes in plant natural product biosynthesis.
- Understanding plant UGTs is vital for harnessing their potential.
Purpose of the Study:
- To provide a comprehensive overview of published plant UGT structures.
- To analyze the catalytic and substrate recognition mechanisms of plant UGTs.
- To summarize and evaluate protein engineering strategies for improving plant UGT activity.
Main Methods:
- Literature review of published plant UGT structures.
- Analysis of catalytic and substrate recognition mechanisms.
- Evaluation of protein engineering techniques, including high-throughput screening.
Main Results:
- Compilation of all available plant UGT structures.
- In-depth analysis of UGT catalytic and substrate binding.
- Summary of protein engineering approaches and their effectiveness.
Conclusions:
- Plant UGTs are critical for natural product synthesis.
- Structural and mechanistic insights guide UGT engineering.
- Advanced screening methods enhance UGT functional improvement.
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