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Simultaneous Determination of General Active Ingredients in Topical Formulations by Gas Chromatography with Flame
Anna Ioutsi1, Anastasia Sarnitskaya1, Mikhail Sumtsov1
1Scientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation, Federal State Budgetary Institution, 8/2 Petrovsky Blvd, Moscow 127051, Russian Federation.
Abstract:
A simple, fast, precision and sensitive gas chromatographic method was developed for determination of camphor, L-menthol, methyl salicylate, salicylic acid, benzyl nicotinate and nonivamide in topical formulations. The possibility of determination low-boiling components and high-boiling components by a single analytical method was shown. The method was optimized by evaluating the effect of a large number of chromatographic parameters and sample preparation stages. It was validated according to the International Council on Harmonization guidelines. Limit of quantification of active components were achieved from 0.7 μg/mL for benzyl nicotinate and L-menthol to 41 μg/mL for salicylic acid. High repeatability and good recovery values were demonstrated. The practical application of the proposed method was shown for ointments and cream.
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