Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

4.9K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
4.9K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

3.3K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.3K
  1. Home
  3. Fxyd3 Functionally Demarcates An Ancestral Breast Cancer Stem Cell Subpopulation With Features Of Drug-tolerant Persisters.
  1. Home
  3. Fxyd3 Functionally Demarcates An Ancestral Breast Cancer Stem Cell Subpopulation With Features Of Drug-tolerant Persisters.

Related Experiment Video

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
07:03

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Published on: October 2, 2020

4.6K

FXYD3 functionally demarcates an ancestral breast cancer stem cell subpopulation with features of drug-tolerant

Mengjiao Li1, Tatsunori Nishimura1, Yasuto Takeuchi1,2

  • 1Division of Cancer Cell Biology, Cancer Research Institute, and.

The Journal of Clinical Investigation
|November 15, 2023

View abstract on PubMed

Summary
This summary is machine-generated.

Cancer stem cells (CSCs) in triple-negative breast cancer (TNBC) show plasticity. A specific subpopulation, FXYD3+ CSCs, drives chemoresistance and may be targeted by Na+/K+ pump inhibitors.

Keywords:
Breast cancerDrug therapyOncologyStem cells

More Related Videos

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.3K
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

10.2K

Related Experiment Videos

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
07:03

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Published on: October 2, 2020

4.6K
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.3K
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

10.2K

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Cancer stem cells (CSCs) contribute to tumor heterogeneity and therapeutic resistance.
  • Phenotypic plasticity in CSCs fuels treatment challenges, particularly in triple-negative breast cancer (TNBC).

Purpose of the Study:

  • To identify and characterize CSC subpopulations driving phenotypic heterogeneity in TNBC.
  • To investigate the role of identified CSC subpopulations in chemoresistance and therapeutic targeting.

Main Methods:

  • Single-cell transcriptomics analysis was performed on TNBC samples.
  • Identification of specific CSC markers and subpopulations.
  • Assessment of FXYD3+ CSC persistence during neoadjuvant chemotherapy.
  • Evaluation of sensitivity to Na+/K+ pump inhibitors.

Main Results:

  • A subpopulation of CSCs, marked by FXYD3 (a Na+/K+ pump component), was identified.
  • FXYD3+ CSCs exhibit ancestral features and traits of alveolar progenitors.
  • These FXYD3+ CSCs persisted through neoadjuvant chemotherapy, acting as drug-tolerant persisters (DTPs).
  • FXYD3+ CSCs demonstrated sensitivity to cardiac glycosides, which are Na+/K+ pump inhibitors.

Conclusions:

  • FXYD3+ CSCs with ancestral features are key drivers of plasticity and chemoresistance in TNBC.
  • Targeting the Na+/K+ pump offers a potential therapeutic strategy to eliminate CSCs and improve TNBC outcomes.
  • Na+/K+ pump inhibitors may effectively target both ancestral and DTP features of CSCs.