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Published on: September 27, 2018
Flow Virometry: A Fluorescence-Based Approach to Enumerate Bacteriophages in Liquid Samples
Elena A Dlusskaya1, Rafik Dey2
1Faculty of Arts, Business & Science, University College of the North, The Pas, MB, Canada. edlusskaya@ucn.ca.
Abstract:
Correctly designed flow cytometry (virometry) assays allow accurate detection and enumeration of viruses in water. However, rigorous controls and calibrators are needed to obtain quality data. In the absence of proper controls, the use of fluorescent dyes for virus enumeration can produce false positive signals and lead to the wrong estimation of total virus counts by misreporting colloid particles as virions. Here we describe a protocol that addresses the problems that might potentially confound virometry data accuracy.
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