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Yolk-Shell Encapsulation of Cells by Biomimetic Mineralization and Visible Light-Induced Surface Graft Polymerization
Kanglei Wang1,2, Changwen Zhao1,2,3, Yuhong Ma4
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Biomacromolecules
|November 15, 2023
Summary
We developed a novel method to create yolk-shell structured yeast cells, enhancing their viability and stability for cell coating applications. This biomimetic approach offers a promising strategy for advanced living cell technologies.
Area of Science:
- Biomaterials Science
- Cell Engineering
- Surface Chemistry
Background:
- Developing low-cytotoxicity cell coating strategies is crucial for practical living cell technologies.
- Existing methods often face challenges in maintaining cell viability and stability.
Purpose of the Study:
- To present a novel method for fabricating yolk-shell structured yeast cells.
- To investigate the impact of this structure on cell viability, lag phase, and storage stability.
Main Methods:
- Biomimetic mineralization to deposit an amorphous calcium carbonate (ACC) shell on yeast cells.
- Surface graft polymerization of poly(ethylene glycol) diacrylate (PEGDA) using visible light.
- Removal of the inner ACC shell to yield yolk-shell structured yeast cells (cell@PHS).
Main Results:
- The cell@PHS retained 98.81% of original cell viability due to mild fabrication conditions.
- The shell thickness tunable lag phase of yeast cells from 5 to 25 hours.
- Cell@PHS demonstrated improved resistance to lyticase and significantly higher viability (81.09%) after 30 days compared to native yeast cells (19.89%).
Conclusions:
- The developed method successfully creates yolk-shell structured yeast cells with high viability.
- The protective shell enhances cell stability, extends lag phase, and improves storage longevity.
- This technique offers a promising platform for advanced cell coating and living cell-related technologies.
