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Related Experiment Video

Updated: Jul 11, 2025

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Visualizing calcium-dependent signaling networks in plants.

Huimin Xu¹, Xinlin Liang², James R Lloyd³

¹State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Trends in Plant Science

|November 15, 2023

Summary

This summary is machine-generated.

Visualizing plant calcium signaling is difficult. A new genetically encoded reporter allows real-time imaging of calcium-dependent protein kinase (CDPK) activity, aiding calcium decoding research.

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Last Updated: Jul 11, 2025

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

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Published on: August 15, 2017

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Published on: September 2, 2014

Area of Science:

Plant biology
Molecular biology
Biochemistry

Background:

Calcium-dependent protein kinases (CDPKs) are crucial regulators in plant signaling pathways.
Directly imaging CDPK activity in living plant cells presents significant technical challenges.

Purpose of the Study:

To introduce a novel genetically encoded reporter for visualizing CDPK activity in real-time.
To enable the study of calcium (Ca2+) dynamics and CDPK conformational changes in plants.

Main Methods:

Development and application of a Förster resonance energy transfer (FRET) based reporter system.
Utilizing genetic encoding for reporter expression within plant cells.

Main Results:

The CDPK-FRET reporter successfully visualizes calcium-dependent conformational changes of CDPKs.
Real-time monitoring of CDPK activation and inactivation in response to calcium signals is achieved.

Conclusions:

The CDPK-FRET reporter is a powerful tool for studying calcium decoding in plants.
This technology facilitates a deeper understanding of CDPK regulatory roles in plant cellular processes.