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Updated: Jul 1, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
Chitin degradation by Synechococcus WH7803
Giovanna Capovilla1, Kurt G Castro2, Silvio Collani3
1Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. gio.capovilla@gmail.com.
Abstract:
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
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