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Updated: Jul 11, 2025
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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
Published on: January 7, 2019
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Stress granules plug and stabilize damaged endolysosomal membranes
Claudio Bussi1, Agustín Mangiarotti2, Christian Vanhille-Campos3,4
1The Francis Crick Institute, London, UK. claudio.bussi@crick.ac.uk.
Nature
|November 15, 2023
Summary
Cells use stress granules to stabilize damaged endomembranes, facilitating repair. Blocking this process aids Mycobacterium tuberculosis survival in macrophages.
Area of Science:
- Cell Biology
- Molecular Biology
- Pathogen Biology
Background:
- Endomembrane damage is a cellular stress impacting eukaryotic cells.
- Cells have repair mechanisms, but stabilization of damaged membranes is poorly understood.
Purpose of the Study:
- Investigate the role of stress granules in stabilizing damaged endomembranes.
- Elucidate the mechanisms of endomembrane repair and their implications for pathogen survival.
Main Methods:
- In vitro and in cellulo studies
- Computational modeling
- Macrophage infection assays
Main Results:
- Stress granules rapidly form at endomembrane damage sites, acting as a plug to stabilize membranes.
- Stress granule formation promotes efficient endolysosome repair via ESCRT-dependent and -independent pathways.
- Inhibition of stress granules enhances Mycobacterium tuberculosis survival in human macrophages.
Conclusions:
- Stress granules are crucial for stabilizing damaged endomembranes and enabling cellular repair.
- Targeting stress granule formation could be a strategy against Mycobacterium tuberculosis infections.
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