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Updated: Jun 20, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
Published on: April 17, 2015
Impact of library input on the hit discovery rate in DNA-encoded chemical library selections
Sara Puglioli1, Sebastian Oehler1, Luca Prati1
1Philochem AG, R&D Department 8112 Otelfingen Switzerland nicholas.favalli@philochem.ch dario.neri@pharma.ethz.ch.
Abstract:
DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 105 copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies.

