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Recognition of Oligonucleotide C by Polydopamine-Coated Solid-State Nanopores
Lei Yang1, Yun-Dong Yin1, Fang-Fang Chen1
1Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Analytical Chemistry
|November 16, 2023
Summary
Polydopamine-coated nanopores enable selective, single-molecule recognition of short oligonucleotides. This advancement aids in distinguishing DNA sequences and lengths for early disease detection.
Area of Science:
- Nanotechnology
- Biomolecular sensing
- Oligonucleotide analysis
Background:
- Single-molecule recognition of short oligonucleotides is crucial for early disease detection.
- Solid-state nanopore sensing offers a platform for high-sensitivity biomolecule analysis.
Purpose of the Study:
- To develop a polydopamine (PDA)-coated nanopore sensing platform for selective oligonucleotide recognition.
- To investigate the interaction mechanisms between PDA-coated nanopores and various oligonucleotide sequences and lengths.
Main Methods:
- Fabrication of solid-state nanopores coated with polydopamine via self-polymerization.
- Single-molecule translocation experiments to measure ionic current blockages.
- Analysis of interactions based on hydrogen bonding forces and translocation rates.
Main Results:
- PDA-coated nanopores selectively recognized PolyC20 over PolyA and PolyT due to distinct hydrogen bonding interactions.
- The platform demonstrated sensitive discrimination of PolyC oligonucleotides with varying lengths (20, 14, and 10 nt).
- PDA functionalization effectively modulated nanopore surface properties, influencing oligonucleotide translocation.
Conclusions:
- Polydopamine functionalization of solid-state nanopores provides a versatile platform for selective oligonucleotide recognition.
- This approach holds promise for developing advanced biosensors for early disease diagnosis.
- Rational design of nanopore surfaces can be achieved for specific biomolecule detection.
