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Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

Lisiane Lappe Dos Reis^1,2, Lirna Salvioni Silva de Souza¹, Francisco Carlos de Oliveira Braga¹

¹Fundação Oswaldo Cruz-Fiocruz, Instituto Leônidas & Maria Deane, Laboratório de Diversidade Microbiana da Amazônia de Importância para a Saúde, Manaus, AM, Brasil.

Memorias Do Instituto Oswaldo Cruz

|November 16, 2023

Abstract

Background:

The parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. Giardia is genotyped into eight assemblages (A-H). Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon and in the world. Due to its zoonotic/zooanthroponotic nature, surveillance initiatives and the definition of Giardia assemblages are important in order to characterise the epidemiological scenario and to implement further control measures.

Objectives:

Determine assemblages of G. duodenalis in sloths from the Brazilian Amazon Region.

Methods:

Faecal parasitological examination of sloths from Amazonas State. Polymerase chain reaction (PCR) targeting the beta giardin (BG), and genes from multilocus sequence typing (MLST) scheme, amplicon sequencing and phylogenetic analysis.

Findings:

Here, we identified, by microscopy, Giardia in two northern sloths (Bradypus tridactylus). These two samples were submitted to molecular assays and it was revealed that both were infected by G. duodenalis assemblage A. Phylogenetic analysis showed that they belong to assemblage A within sequences from humans and wild and domestic animals.

Conclusion:

Therefore, besides showing, by the first time, the current presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic/zooanthroponotic scenario of this parasite in the Brazilian Amazon.

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